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- Title
Imaging GABAc receptors with ligand-conjugated quantum dots.
- Authors
Tomlinson, Ian D; Gussin, Hélène A; Little, Deborah M; Warnement, Michael R; Qian, Haohua; Pepperberg, David R; Rosenthal, Sandra J
- Abstract
We report a methodology for labeling the GABAc receptor on the surface membrane of intact cells. This work builds upon our earlier work with serotonin-conjugated quantum dots and our studies with PEGylated quantum dots to reduce nonspecific binding. In the current approach, a PEGylated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphilic polymer derivative of a modified polyacrylamide. These conjugates were used to image GABA(C) receptors heterologously expressed in Xenopus laevis oocytes.
- Publication
Journal of biomedicine & biotechnology, 2007, Vol 2007, p76514
- ISSN
1110-7243
- Publication type
Journal Article
- DOI
10.1155/2007/76514