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- Title
Quasimonomorphic mononucleotide repeats for high-level microsatellite instability analysis.
- Authors
Buhard, Olivier; Suraweera, Nirosha; Lectard, Aude; Duval, Alex; Hamelin, Richard
- Abstract
Microsatellite instability (MSI) analysis is becoming more and more important to detect sporadic primary tumors of the MSI phenotype as well as in helping to determine Hereditary Non-Polyposis Colorectal Cancer (HNPCC) cases. After some years of conflicting data due to the absence of consensus markers for the MSI phenotype, a meeting held in Bethesda to clarify the situation proposed a set of 5 microsatellites (2 mononucleotide repeats and 3 dinucleotide repeats) to determine MSI tumors. A second Bethesda consensus meeting was held at the end of 2002. It was discussed here that the 1998 microsatellite panel could underestimate high-level MSI tumors and overestimate low-level MSI tumors. Amongst the suggested changes was the exclusive use of mononucleotide repeats in place of dinucleotide repeats. We have already proposed a pentaplex MSI screening test comprising 5 quasimonomorphic mononucleotide repeats. This article compares the advantages of mono or dinucleotide repeats in determining microsatellite instability.
- Publication
Disease markers, 2004, Vol 20, Issue 4-5, p251
- ISSN
0278-0240
- Publication type
Journal Article
- DOI
10.1155/2004/159347