We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The genetics and genomics of the silkworm, Bombyx mori.
- Authors
Goldsmith, Marian R; Shimada, Toru; Abe, Hiroaki
- Abstract
We review progress in applying molecular genetic and genomic technologies to studies in the domesticated silkworm, Bombyx mori, highlighting its use as a model for Lepidoptera, and in sericulture and biotechnology. Dense molecular linkage maps are being integrated with classical linkage maps for positional cloning and marker-assisted selection. Classical mutations have been identified by a candidate gene approach. Cytogenetic and sequence analyses show that the W chromosome is composed largely of nested full-length long terminal repeat retrotransposons. Z-chromosome-linked sequences show a lack of dosage compensation. The downstream sex differentiation mechanism has been studied via the silkworm homolog of doublesex. Expressed sequence tagged databases have been used to discover Lepidoptera-specific genes, provide evidence for horizontal gene transfer, and construct microarrays. Physical maps using large-fragment bacterial artificial chromosome libraries have been constructed, and whole-genome shotgun sequencing is underway. Germline transformation and transient expression systems are well established and available for functional studies, high-level protein expression, and gene silencing via RNA interference.
- Publication
Annual review of entomology, 2005, Vol 50, p71
- ISSN
0066-4170
- Publication type
Journal Article
- DOI
10.1146/annurev.ento.50.071803.130456