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- Title
Two-photon excitation fluorescence microscopy.
- Authors
So, P T; Dong, C Y; Masters, B R; Berland, K M
- Abstract
Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.
- Publication
Annual review of biomedical engineering, 2000, Vol 2, p399
- ISSN
1523-9829
- Publication type
Journal Article
- DOI
10.1146/annurev.bioeng.2.1.399