We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Pig cloning by microinjection of fetal fibroblast nuclei.
- Authors
Onishi, A; Iwamoto, M; Akita, T; Mikawa, S; Takeda, K; Awata, T; Hanada, H; Perry, A C
- Abstract
Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.
- Publication
Science (New York, N.Y.), 2000, Vol 289, Issue 5482, p1188
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.289.5482.1188