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- Title
Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels.
- Authors
Gulbis, J M; Zhou, M; Mann, S; MacKinnon, R
- Abstract
The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane alpha subunit together with the oxidoreductase beta subunit in a fourfold symmetric T1(4)beta4 complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four beta subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T1(4)beta4 complex. The inactivation peptides of voltage-dependent K(+) channels reach their site of action by entering these openings.
- Publication
Science (New York, N.Y.), 2000, Vol 289, Issue 5476, p123
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.289.5476.123