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- Title
Translating biomolecular recognition into nanomechanics.
- Authors
Fritz, J; Baller, M K; Lang, H P; Rothuizen, H; Vettiger, P; Meyer, E; Güntherodt, H; Gerber, C; Gimzewski, J K
- Abstract
We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.
- Publication
Science (New York, N.Y.), 2000, Vol 288, Issue 5464, p316
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.288.5464.316