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- Title
A BAC-based physical map of the major autosomes of Drosophila melanogaster.
- Authors
Hoskins, R A; Nelson, C R; Berman, B P; Laverty, T R; George, R A; Ciesiolka, L; Naeemuddin, M; Arenson, A D; Durbin, J; David, R G; Tabor, P E; Bailey, M R; DeShazo, D R; Catanese, J; Mammoser, A; Osoegawa, K; de Jong, P J; Celniker, S E; Gibbs, R A; Rubin, G M; Scherer, S E
- Abstract
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.
- Publication
Science (New York, N.Y.), 2000, Vol 287, Issue 5461, p2271
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.287.5461.2271