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- Title
Start sites of bidirectional DNA synthesis at the human lamin B2 origin.
- Authors
Abdurashidova, G; Deganuto, M; Klima, R; Riva, S; Biamonti, G; Giacca, M; Falaschi, A
- Abstract
The initiation sites of bidirectional synthesis at the DNA replication origin located at the 3' end of the human lamin B2 gene were investigated. RNA-primed nascent DNA molecules were subjected to second-strand synthesis with appropriate primers, amplified by ligation-mediated polymerase chain reaction, and size fractionated. Evidence for precise start sites was obtained. Exploration of close to 1 kilobase, coupled to inhibition of Okazaki fragment synthesis, demonstrates that the leading strands initiate at precise nucleotides on either helix, overlapping by three base pairs, within the area bound to a protein complex possibly analogous to the prereplicative complex of yeast.
- Publication
Science (New York, N.Y.), 2000, Vol 287, Issue 5460, p2023
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.287.5460.2023