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- Title
A biochemical genomics approach for identifying genes by the activity of their products.
- Authors
Martzen, M R; McCraith, S M; Spinelli, S L; Torres, F M; Fields, S; Grayhack, E J; Phizicky, E M
- Abstract
For the identification of yeast genes specifying biochemical activities, a genomic strategy that is rapid, sensitive, and widely applicable was developed with an array of 6144 individual yeast strains, each containing a different yeast open reading frame (ORF) fused to glutathione S-transferase (GST). For the identification of ORF-associated activities, strains were grown in defined pools, and GST-ORFs were purified. Then, pools were assayed for activities, and active pools were deconvoluted to identify the source strains. Three previously unknown ORF-associated activities were identified with this strategy: a cyclic phosphodiesterase that acts on adenosine diphosphate-ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p-processing activity, and a cytochrome c methyltransferase.
- Publication
Science (New York, N.Y.), 1999, Vol 286, Issue 5442, p1153
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.286.5442.1153