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- Title
Whole-genome shotgun optical mapping of Deinococcus radiodurans.
- Authors
Lin, J; Qi, R; Aston, C; Jing, J; Anantharaman, T S; Mishra, B; White, O; Daly, M J; Minton, K W; Venter, J C; Schwartz, D C
- Abstract
A whole-genome restriction map of Deinococcus radiodurans, a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases) was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after gamma irradiation.
- Publication
Science (New York, N.Y.), 1999, Vol 285, Issue 5433, p1558
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.285.5433.1558