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- Title
Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
- Authors
Shi, S H; Hayashi, Y; Petralia, R S; Zaman, S H; Wenthold, R J; Svoboda, K; Malinow, R
- Abstract
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.
- Publication
Science (New York, N.Y.), 1999, Vol 284, Issue 5421, p1811
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.284.5421.1811