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- Title
Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.
- Authors
Skowyra, D; Koepp, D M; Kamura, T; Conrad, M N; Conaway, R C; Conaway, J W; Elledge, S J; Harper, J W
- Abstract
Control of cyclin levels is critical for proper cell cycle regulation. In yeast, the stability of the G1 cyclin Cln1 is controlled by phosphorylation-dependent ubiquitination. Here it is shown that this reaction can be reconstituted in vitro with an SCF E3 ubiquitin ligase complex. Phosphorylated Cln1 was ubiquitinated by SCF (Skp1-Cdc53-F-box protein) complexes containing the F-box protein Grr1, Rbx1, and the E2 Cdc34. Rbx1 promotes association of Cdc34 with Cdc53 and stimulates Cdc34 auto-ubiquitination in the context of Cdc53 or SCF complexes. Rbx1, which is also a component of the von Hippel-Lindau tumor suppressor complex, may define a previously unrecognized class of E3-associated proteins.
- Publication
Science (New York, N.Y.), 1999, Vol 284, Issue 5414, p662
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.284.5414.662