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- Title
Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Poleta.
- Authors
Johnson, R E; Prakash, S; Prakash, L
- Abstract
The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase eta.
- Publication
Science (New York, N.Y.), 1999, Vol 283, Issue 5404, p1001
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.283.5404.1001