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- Title
Yeast Ku as a regulator of chromosomal DNA end structure.
- Authors
Gravel, S; Larrivée, M; Labrecque, P; Wellinger, R J
- Abstract
During telomere replication in yeast, chromosome ends acquire an S-phase-specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross-linking experiments demonstrated that Ku is bound to telomeric DNA. These results show that Ku plays a direct role in establishing a normal DNA end structure on yeast chromosomes, conceivably by functioning as a terminus-binding factor. Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres.
- Publication
Science (New York, N.Y.), 1998, Vol 280, Issue 5364, p741
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.280.5364.741