We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Conservation of substrate recognition mechanisms by tRNA splicing endonucleases.
- Authors
Fabbri, S; Fruscoloni, P; Bufardeci, E; Di Nicola Negri, E; Baldi, M I; Attardi, D G; Mattoccia, E; Tocchini-Valentini, G P
- Abstract
Accuracy in transfer RNA (tRNA) splicing is essential for the formation of functional tRNAs, and hence for gene expression, in both Eukaryotes and Archaea. The specificity for recognition of the tRNA precursor (pre-tRNA) resides in the endonuclease, which removes the intron by making two independent endonucleolytic cleavages. Although the eukaryal and archaeal enzymes appear to use different features of pre-tRNAs to determine the sites of cleavage, analysis of hybrid pre-tRNA substrates containing eukaryal and archaeal sequences, described here, reveals that the eukaryal enzyme retains the ability to use the archaeal recognition signals. This result indicates that there may be a common ancestral mechanism for recognition of pre-tRNA by proteins.
- Publication
Science (New York, N.Y.), 1998, Vol 280, Issue 5361, p284
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.280.5361.284