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- Title
Sphingosine-1-phosphate as a ligand for the G protein-coupled receptor EDG-1.
- Authors
Lee, M J; Van Brocklyn, J R; Thangada, S; Liu, C H; Hand, A R; Menzeleev, R; Spiegel, S; Hla, T
- Abstract
The sphingolipid metabolite sphingosine-1-phosphate (SPP) has been implicated as a second messenger in cell proliferation and survival. However, many of its biological effects are due to binding to unidentified receptors on the cell surface. SPP activated the heterotrimeric guanine nucleotide binding protein (G protein)-coupled orphan receptor EDG-1, originally cloned as Endothelial Differentiation Gene-1. EDG-1 bound SPP with high affinity (dissociation constant = 8.1 nM) and high specificity. Overexpression of EDG-1 induced exaggerated cell-cell aggregation, enhanced expression of cadherins, and formation of well-developed adherens junctions in a manner dependent on SPP and the small guanine nucleotide binding protein Rho.
- Publication
Science (New York, N.Y.), 1998, Vol 279, Issue 5356, p1552
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.279.5356.1552