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- Title
IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation.
- Authors
Mercurio, F; Zhu, H; Murray, B W; Shevchenko, A; Bennett, B L; Li, J; Young, D B; Barbosa, M; Mann, M; Manning, A; Rao, A
- Abstract
Activation of the transcription factor nuclear factor kappa B (NF-kappaB) is controlled by sequential phosphorylation, ubiquitination, and degradation of its inhibitory subunit IkappaB. A large multiprotein complex, the IkappaB kinase (IKK) signalsome, was purified from HeLa cells and found to contain a cytokine-inducible IkappaB kinase activity that phosphorylates IkappaB-alpha and IkappaB-beta. Two components of the IKK signalsome, IKK-1 and IKK-2, were identified as closely related protein serine kinases containing leucine zipper and helix-loop-helix protein interaction motifs. Mutant versions of IKK-2 had pronounced effects on RelA nuclear translocation and NF-kappaB-dependent reporter activity, consistent with a critical role for the IKK kinases in the NF-kappaB signaling pathway.
- Publication
Science (New York, N.Y.), 1997, Vol 278, Issue 5339, p860
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.278.5339.860