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- Title
Stabilization of beta-catenin by genetic defects in melanoma cell lines.
- Authors
Rubinfeld, B; Robbins, P; El-Gamil, M; Albert, I; Porfiri, E; Polakis, P
- Abstract
Signal transduction by beta-catenin involves its posttranslational stabilization and downstream coupling to the Lef and Tcf transcription factors. Abnormally high amounts of beta-catenin were detected in 7 of 26 human melanoma cell lines. Unusual messenger RNA splicing and missense mutations in the beta-catenin gene (CTNNB1) that result in stabilization of the protein were identified in six of the lines, and the adenomatous polyposis coli tumor suppressor protein (APC) was altered or missing in two others. In the APC-deficient cells, ectopic expression of wild-type APC eliminated the excess beta-catenin. Cells with stabilized beta-catenin contained a constitutive beta-catenin-Lef-1 complex. Thus, genetic defects that result in up-regulation of beta-catenin may play a role in melanoma progression.
- Publication
Science (New York, N.Y.), 1997, Vol 275, Issue 5307, p1790
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.275.5307.1790