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- Title
Stereoselective bimolecular phenoxy radical coupling by an auxiliary (dirigent) protein without an active center.
- Authors
Davin, L B; Wang, H B; Crowell, A L; Bedgar, D L; Martin, D M; Sarkanen, S; Lewis, N G
- Abstract
The regio- and stereospecificity of bimolecular phenoxy radical coupling reactions, of especial importance in lignin and lignan biosynthesis, are clearly controlled in some manner in vivo; yet in vitro coupling by oxidases, such as laccases, only produce racemic products. In other words, laccases, peroxidases, and comparable oxidases are unable to control regio- or stereospecificity by themselves and thus some other agent must exist. A 78-kilodalton protein has been isolated that, in the presence of an oxidase or one electron oxidant, effects stereoselective bimolecular phenoxy radical coupling in vitro. Itself lacking a catalytically active (oxidative) center, its mechanism of action is presumed to involve capture of E-coniferyl alcohol-derived free-radical intermediates, with consequent stereoselective coupling to give (+)-pinoresinol.
- Publication
Science (New York, N.Y.), 1997, Vol 275, Issue 5298, p362
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.275.5298.362