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- Title
Nucleosome arrays reveal the two-start organization of the chromatin fiber.
- Authors
Dorigo, Benedetta; Schalch, Thomas; Kulangara, Alexandra; Duda, Sylwia; Schroeder, Rasmus R; Richmond, Timothy J
- Abstract
Chromatin folding determines the accessibility of DNA constituting eukaryotic genomes and consequently is profoundly important in the mechanisms of nuclear processes such as gene regulation. Nucleosome arrays compact to form a 30-nanometer chromatin fiber of hitherto disputed structure. Two competing classes of models have been proposed in which nucleosomes are either arranged linearly in a one-start higher order helix or zigzag back and forth in a two-start helix. We analyzed compacted nucleosome arrays stabilized by introduction of disulfide cross-links and show that the chromatin fiber comprises two stacks of nucleosomes in accord with the two-start model.
- Publication
Science (New York, N.Y.), 2004, Vol 306, Issue 5701, p1571
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1103124