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- Title
No transcription-translation feedback in circadian rhythm of KaiC phosphorylation.
- Authors
Tomita, Jun; Nakajima, Masato; Kondo, Takao; Iwasaki, Hideo
- Abstract
An autoregulatory transcription-translation feedback loop is thought to be essential in generating circadian rhythms in any model organism. In the cyanobacterium Synechococcus elongatus, the essential clock protein KaiC is proposed to form this type of transcriptional negative feedback. Nevertheless, we demonstrate here temperature-compensated, robust circadian cycling of KaiC phosphorylation even without kaiBC messenger RNA accumulation under continuous dark conditions. This rhythm persisted in the presence of a transcription or translation inhibitor. Moreover, kinetic profiles in the ratio of KaiC autophosphorylation-dephosphorylation were also temperature compensated in vitro. Thus, the cyanobacterial clock can keep time independent of de novo transcription and translation processes.
- Publication
Science (New York, N.Y.), 2005, Vol 307, Issue 5707, p251
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1102540