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- Title
Crystal structure of a shark single-domain antibody V region in complex with lysozyme.
- Authors
Stanfield, Robyn L; Dooley, Helen; Flajnik, Martin F; Wilson, Ian A
- Abstract
Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
- Publication
Science (New York, N.Y.), 2004, Vol 305, Issue 5691, p1770
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1101148