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- Title
Activation of endogenous Cdc42 visualized in living cells.
- Authors
Nalbant, Perihan; Hodgson, Louis; Kraynov, Vadim; Toutchkine, Alexei; Hahn, Klaus M
- Abstract
Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.
- Publication
Science (New York, N.Y.), 2004, Vol 305, Issue 5690, p1615
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1100367