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- Title
Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers.
- Authors
Shorter, James; Lindquist, Susan
- Abstract
The protein-remodeling factor Hsp104 governs inheritance of [PSI+], a yeast prion formed by self-perpetuating amyloid conformers of the translation termination factor Sup35. Perplexingly, either excess or insufficient Hsp104 eliminates [PSI+]. In vitro, at low concentrations, Hsp104 catalyzed the formation of oligomeric intermediates that proved critical for the nucleation of Sup 35 fibrillization de novo and displayed a conformation common among amyloidogenic polypeptides. At higher Hsp104 concentrations, amyloidogenic oligomerization and contingent fibrillization were abolished. Hsp104 also disassembled mature fibers in a manner that initially exposed new surfaces for conformational replication but eventually exterminated prion conformers. These Hsp104 activities differed in their reaction mechanism and can explain [PSI+] inheritance patterns.
- Publication
Science (New York, N.Y.), 2004, Vol 304, Issue 5678, p1793
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1098007