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- Title
Discovery and directed evolution of a glyphosate tolerance gene.
- Authors
Castle, Linda A; Siehl, Daniel L; Gorton, Rebecca; Patten, Phillip A; Chen, Yong Hong; Bertain, Sean; Cho, Hyeon-Je; Duck, Nicholas; Wong, James; Liu, Donglong; Lassner, Michael W
- Abstract
The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.
- Publication
Science (New York, N.Y.), 2004, Vol 304, Issue 5674, p1151
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1096770