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- Title
Stathmin-tubulin interaction gradients in motile and mitotic cells.
- Authors
Niethammer, Philipp; Bastiaens, Philippe; Karsenti, Eric
- Abstract
The spatial organization of the microtubule cytoskeleton is thought to be directed by steady-state activity gradients of diffusible regulatory molecules. We visualized such intracellular gradients by monitoring the interaction between tubulin and a regulator of microtubule dynamics, stathmin, using a fluorescence resonance energy transfer (FRET) biosensor. These gradients were observed both during interphase in motile membrane protrusions and during mitosis around chromosomes, which suggests that a similar mechanism may contribute to the creation of polarized microtubule structures. These interaction patterns are likely to reflect phosphorylation of stathmin in these areas.
- Publication
Science (New York, N.Y.), 2004, Vol 303, Issue 5665, p1862
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1094108