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- Title
Genome-wide RNAi analysis of growth and viability in Drosophila cells.
- Authors
Boutros, Michael; Kiger, Amy A; Armknecht, Susan; Kerr, Kim; Hild, Marc; Koch, Britta; Haas, Stefan A; Paro, Renato; Perrimon, Norbert; Heidelberg Fly Array Consortium
- Abstract
A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.
- Publication
Science (New York, N.Y.), 2004, Vol 303, Issue 5659, p832
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1091266