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- Title
Nuclear export of microRNA precursors.
- Authors
Lund, Elsebet; Güttinger, Stephan; Calado, Angelo; Dahlberg, James E; Kutay, Ulrike
- Abstract
MicroRNAs (miRNAs), which function as regulators of gene expression in eukaryotes, are processed from larger transcripts by sequential action of nuclear and cytoplasmic ribonuclease III-like endonucleases. We show that Exportin-5 (Exp5) mediates efficient nuclear export of short miRNA precursors (pre-miRNAs) and that its depletion by RNA interference results in reduced miRNA levels. Exp5 binds correctly processed pre-miRNAs directly and specifically, in a Ran guanosine triphosphate-dependent manner, but interacts only weakly with extended pre-miRNAs that yield incorrect miRNAs when processed by Dicer in vitro. Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps.
- Publication
Science (New York, N.Y.), 2004, Vol 303, Issue 5654, p95
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1090599