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- Title
A protein interaction map of Drosophila melanogaster.
- Authors
Giot, L; Bader, J S; Brouwer, C; Chaudhuri, A; Kuang, B; Li, Y; Hao, Y L; Ooi, C E; Godwin, B; Vitols, E; Vijayadamodar, G; Pochart, P; Machineni, H; Welsh, M; Kong, Y; Zerhusen, B; Malcolm, R; Varrone, Z; Collis, A; Minto, M; Burgess, S; McDaniel, L; Stimpson, E; Spriggs, F; Williams, J; Neurath, K; Ioime, N; Agee, M; Voss, E; Furtak, K; Renzulli, R; Aanensen, N; Carrolla, S; Bickelhaupt, E; Lazovatsky, Y; DaSilva, A; Zhong, J; Stanyon, C A; Finley, R L, Jr; White, K P; Braverman, M; Jarvie, T; Gold, S; Leach, M; Knight, J; Shimkets, R A; McKenna, M P; Chant, J; Rothberg, J M
- Abstract
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.
- Publication
Science (New York, N.Y.), 2003, Vol 302, Issue 5651, p1727
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1090289