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- Title
Design of a novel globular protein fold with atomic-level accuracy.
- Authors
Kuhlman, Brian; Dantas, Gautam; Ireton, Gregory C; Varani, Gabriele; Stoddard, Barry L; Baker, David
- Abstract
A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature.
- Publication
Science (New York, N.Y.), 2003, Vol 302, Issue 5649, p1364
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1089427