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- Title
Empirical analysis of transcriptional activity in the Arabidopsis genome.
- Authors
Yamada, Kayoko; Lim, Jun; Dale, Joseph M; Chen, Huaming; Shinn, Paul; Palm, Curtis J; Southwick, Audrey M; Wu, Hank C; Kim, Christopher; Nguyen, Michelle; Pham, Paul; Cheuk, Rosa; Karlin-Newmann, George; Liu, Shirley X; Lam, Bao; Sakano, Hitomi; Wu, Troy; Yu, Guixia; Miranda, Molly; Quach, Hong L; Tripp, Matthew; Chang, Charlie H; Lee, Jeong M; Toriumi, Mitsue; Chan, Marie M H; Tang, Carolyn C; Onodera, Courtney S; Deng, Justine M; Akiyama, Kenji; Ansari, Yasser; Arakawa, Takahiro; Banh, Jenny; Banno, Fumika; Bowser, Leah; Brooks, Shelise; Carninci, Piero; Chao, Qimin; Choy, Nathan; Enju, Akiko; Goldsmith, Andrew D; Gurjal, Mani; Hansen, Nancy F; Hayashizaki, Yoshihide; Johnson-Hopson, Chanda; Hsuan, Vickie W; Iida, Kei; Karnes, Meagan; Khan, Shehnaz; Koesema, Eric; Ishida, Junko; Jiang, Paul X; Jones, Ted; Kawai, Jun; Kamiya, Asako; Meyers, Cristina; Nakajima, Maiko; Narusaka, Mari; Seki, Motoaki; Sakurai, Tetsuya; Satou, Masakazu; Tamse, Racquel; Vaysberg, Maria; Wallender, Erika K; Wong, Cecilia; Yamamura, Yuki; Yuan, Shiaulou; Shinozaki, Kazuo; Davis, Ronald W; Theologis, Athanasios; Ecker, Joseph R
- Abstract
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.
- Publication
Science (New York, N.Y.), 2003, Vol 302, Issue 5646, p842
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1088305