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- Title
Nuclear membrane proteins with potential disease links found by subtractive proteomics.
- Authors
Schirmer, Eric C; Florens, Laurence; Guan, Tinglu; Yates, John R, 3rd; Gerace, Larry
- Abstract
To comprehensively identify integral membrane proteins of the nuclear envelope (NE), we prepared separately NEs and organelles known to cofractionate with them from liver. Proteins detected by multidimensional protein identification technology in the cofractionating organelles were subtracted from the NE data set. In addition to all 13 known NE integral proteins, 67 uncharacterized open reading frames with predicted membrane-spanning regions were identified. All of the eight proteins tested targeted to the NE, indicating that there are substantially more integral proteins of the NE than previously thought. Furthermore, 23 of these mapped within chromosome regions linked to a variety of dystrophies.
- Publication
Science (New York, N.Y.), 2003, Vol 301, Issue 5638, p1380
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1088176