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- Title
Sequence-dependent pausing of single lambda exonuclease molecules.
- Authors
Perkins, Thomas T; Dalal, Ravindra V; Mitsis, Paul G; Block, Steven M
- Abstract
Lambda exonuclease processively degrades one strand of duplex DNA, moving 5'-to-3' in an ATP-independent fashion. When examined at the single-molecule level, the speeds of digestion were nearly constant at 4 nanometers per second (12 nucleotides per second), interspersed with pauses of variable duration. Long pauses, occurring at stereotypical locations, were strand-specific and sequence-dependent. Pause duration and probability varied widely. The strongest pause, GGCGAT TCT, was identified by gel electrophoresis. Correlating single-molecule dwell positions with sequence independently identified the motif GGCGA. This sequence is found in the left lambda cohesive end, where exonuclease inhibition may contribute to the reduced recombination efficiency at that end.
- Publication
Science (New York, N.Y.), 2003, Vol 301, Issue 5641, p1914
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1088047