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- Title
Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli.
- Authors
Huang, Yafei; Lemieux, M Joanne; Song, Jinmei; Auer, Manfred; Wang, Da-Neng
- Abstract
The major facilitator superfamily represents the largest group of secondary membrane transporters in the cell. Here we report the 3.3 angstrom resolution structure of a member of this superfamily, GlpT, which transports glycerol-3-phosphate into the cytoplasm and inorganic phosphate into the periplasm. The amino- and carboxyl-terminal halves of the protein exhibit a pseudo two-fold symmetry. Closed off to the periplasm, a centrally located substrate-translocation pore contains two arginines at its closed end, which comprise the substrate-binding site. Upon substrate binding, the protein adopts a more compact conformation. We propose that GlpT operates by a single-binding site, alternating-access mechanism through a rocker-switch type of movement.
- Publication
Science (New York, N.Y.), 2003, Vol 301, Issue 5633, p616
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1087619