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- Title
Structural basis of a phototropin light switch.
- Authors
Harper, Shannon M; Neil, Lori C; Gardner, Kevin H
- Abstract
Phototropins are light-activated kinases important for plant responses to blue light. Light initiates signaling in these proteins by generating a covalent protein-flavin mononucleotide (FMN) adduct within sensory Per-ARNT-Sim (PAS) domains. We characterized the light-dependent changes of a phototropin PAS domain by solution nuclear magnetic resonance spectroscopy and found that an alpha helix located outside the canonical domain plays a key role in this activation process. Although this helix associates with the PAS core in the dark, photoinduced changes in the domain structure disrupt this interaction. We propose that this mechanism couples light-dependent bond formation to kinase activation and identifies a signaling pathway conserved among PAS domains.
- Publication
Science (New York, N.Y.), 2003, Vol 301, Issue 5639, p1541
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1086810