We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reprogramming control of an allosteric signaling switch through modular recombination.
- Authors
Dueber, John E; Yeh, Brian J; Chak, Kayam; Lim, Wendell A
- Abstract
Many eukaryotic signaling proteins are composed of simple modular binding domains, yet they can display sophisticated behaviors such as allosteric gating and multi-input signal integration, properties essential for complex cellular circuits. To understand how such behavior can emerge from combinations of simple domains, we engineered variants of the actin regulatory protein N-WASP (neuronal Wiskott-Aldrich syndrome protein) in which the "output" domain of N-WASP was recombined with heterologous autoinhibitory "input" domains. Synthetic switch proteins were created with diverse gating behaviors in response to nonphysiological inputs. Thus, this type of modular framework can facilitate the evolution or engineering of cellular signaling circuits.
- Publication
Science (New York, N.Y.), 2003, Vol 301, Issue 5641, p1904
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1085945