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- Title
Single molecule profiling of alternative pre-mRNA splicing.
- Authors
Zhu, Jun; Shendure, Jay; Mitra, Robi D; Church, George M
- Abstract
Alternative pre-messenger RNA splicing is an important mechanism for generating protein diversity and may explain in part how mammalian complexity arises from a surprisingly small complement of genes. Here, we describe "digital polony exon profiling,"a single molecule-based technology for studying complex alternative pre-messenger RNA splicing. This technology allows researchers to monitor the combinatorial diversity of exon inclusion in individual transcripts. A minisequencing strategy provides single nucleotide resolution, and the digital nature of the technology allows quantitation of individual splicing variants. Digital polony exon profiling can be used to investigate the physiological and pathological roles of alternately spliced messenger RNAs, as well as the mechanisms by which these messenger RNAs are produced.
- Publication
Science (New York, N.Y.), 2003, Vol 301, Issue 5634, p836
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1085792