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- Title
Programmed DNA deletion as an RNA-guided system of genome defense.
- Authors
Yao, Meng-Chao; Fuller, Patrick; Xi, Xiaohui
- Abstract
Genomewide DNA rearrangements occur in many eukaryotes during development, but their functions and mechanisms are poorly understood. Previous studies have implicated a sequence-recognition mechanism based on RNA-mediated interactions between nuclei in ciliated protozoa. In this study, we found that the process recognized and deleted a foreign gene integrated in a Tetrahymena chromosome, suggesting an unusual mechanism of genome surveillance. We further found that injection of double-stranded RNA into the cell at specific developmental stages triggers efficient deletion of the targeted genomic regions. Together the results indicate an RNA-based mechanism that directs genomewide DNA rearrangements and serves to disable invading genetic agents.
- Publication
Science (New York, N.Y.), 2003, Vol 300, Issue 5625, p1581
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1084737