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- Title
Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization.
- Authors
Yildiz, Ahmet; Forkey, Joseph N; McKinney, Sean A; Ha, Taekjip; Goldman, Yale E; Selvin, Paul R
- Abstract
Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving approximately 37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of <1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 - 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.
- Publication
Science (New York, N.Y.), 2003, Vol 300, Issue 5628, p2061
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1084398