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- Title
Crystal structure of the carboxyltransferase domain of acetyl-coenzyme A carboxylase.
- Authors
Zhang, Hailong; Yang, Zhiru; Shen, Yang; Tong, Liang
- Abstract
Acetyl-coenzyme A carboxylases (ACCs) are required for the biosynthesis and oxidation of long-chain fatty acids. They are targets for therapeutics against obesity and diabetes, and several herbicides function by inhibiting their carboxyltransferase (CT) domain. We determined the crystal structure of the free enzyme and the coenzyme A complex of yeast CT at 2.7 angstrom resolution and found that it comprises two domains, both belonging to the crotonase/ClpP superfamily. The active site is at the interface of a dimer. Mutagenesis and kinetic studies reveal the functional roles of conserved residues here. The herbicides target the active site of CT, providing a lead for inhibitor development against human ACCs.
- Publication
Science (New York, N.Y.), 2003, Vol 299, Issue 5615, p2064
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1081366