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- Title
Myc-induced T cell leukemia in transgenic zebrafish.
- Authors
Langenau, David M; Traver, David; Ferrando, Adolfo A; Kutok, Jeffery L; Aster, Jon C; Kanki, John P; Lin, Shuo; Prochownik, Ed; Trede, Nikolaus S; Zon, Leonard I; Look, A Thomas
- Abstract
The zebrafish is an attractive model organism for studying cancer development because of its genetic accessibility. Here we describe the induction of clonally derived T cell acute lymphoblastic leukemia in transgenic zebrafish expressing mouse c-myc under control of the zebrafish Rag2 promoter. Visualization of leukemic cells expressing a chimeric transgene encoding Myc fused to green fluorescent protein (GFP) revealed that leukemias arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into skeletal muscle and abdominal organs. Leukemic cells homed back to the thymus in irradiated fish transplanted with GFP-labeled leukemic lymphoblasts. This transgenic model provides a platform for drug screens and for genetic screens aimed at identifying mutations that suppress or enhance c-myc- induced carcinogenesis.
- Publication
Science (New York, N.Y.), 2003, Vol 299, Issue 5608, p887
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1080280