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- Title
Zero-mode waveguides for single-molecule analysis at high concentrations.
- Authors
Levene, M J; Korlach, J; Turner, S W; Foquet, M; Craighead, H G; Webb, W W
- Abstract
Optical approaches for observing the dynamics of single molecules have required pico- to nanomolar concentrations of fluorophore in order to isolate individual molecules. However, many biologically relevant processes occur at micromolar ligand concentrations, necessitating a reduction in the conventional observation volume by three orders of magnitude. We show that arrays of zero-mode waveguides consisting of subwavelength holes in a metal film provide a simple and highly parallel means for studying single-molecule dynamics at micromolar concentrations with microsecond temporal resolution. We present observations of DNA polymerase activity as an example of the effectiveness of zero-mode waveguides for performing single-molecule experiments at high concentrations.
- Publication
Science (New York, N.Y.), 2003, Vol 299, Issue 5607, p682
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1079700