We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation.
- Authors
Zilberman, Daniel; Cao, Xiaofeng; Jacobsen, Steven E
- Abstract
Proteins of the ARGONAUTE family are important in diverse posttranscriptional RNA-mediated gene-silencing systems as well as in transcriptional gene silencing in Drosophila and fission yeast and in programmed DNA elimination in Tetrahymena. We cloned ARGONAUTE4 (AGO4) from a screen for mutants that suppress silencing of the Arabidopsis SUPERMAN (SUP) gene. The ago4-1 mutant reactivated silent SUP alleles and decreased CpNpG and asymmetric DNA methylation as well as histone H3 lysine-9 methylation. In addition, ago4-1 blocked histone and DNA methylation and the accumulation of 25-nucleotide small interfering RNAs (siRNAs) that correspond to the retroelement AtSN1. These results suggest that AGO4 and long siRNAs direct chromatin modifications, including histone methylation and non-CpG DNA methylation.
- Publication
Science (New York, N.Y.), 2003, Vol 299, Issue 5607, p716
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1079695