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- Title
Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum.
- Authors
Foth, Bernardo J; Ralph, Stuart A; Tonkin, Christopher J; Struck, Nicole S; Fraunholz, Martin; Roos, David S; Cowman, Alan F; McFadden, Geoffrey I
- Abstract
Transit peptides mediate protein targeting into plastids and are only poorly understood. We extracted amino acid features from transit peptides that target proteins to the relict plastid (apicoplast) of malaria parasites. Based on these amino acid characteristics, we identified 466 putative apicoplast proteins in the Plasmodium falciparum genome. Altering the specific charge characteristics in a model transit peptide by site-directed mutagenesis severely disrupted organellar targeting in vivo. Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting.
- Publication
Science (New York, N.Y.), 2003, Vol 299, Issue 5607, p705
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1078599