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- Title
Structural basis of transcription activation: the CAP-alpha CTD-DNA complex.
- Authors
Benoff, Brian; Yang, Huanwang; Lawson, Catherine L; Parkinson, Gary; Liu, Jinsong; Blatter, Erich; Ebright, Yon W; Berman, Helen M; Ebright, Richard H
- Abstract
The Escherichia coli catabolite activator protein (CAP) activates transcription at P(lac), P(gal), and other promoters through interactions with the RNA polymerase alpha subunit carboxyl-terminal domain (alphaCTD). We determined the crystal structure of the CAP-alphaCTD-DNA complex at a resolution of 3.1 angstroms. CAP makes direct protein-protein interactions with alphaCTD, and alphaCTD makes direct protein-DNA interactions with the DNA segment adjacent to the DNA site for CAP. There are no large-scale conformational changes in CAP and alphaCTD, and the interface between CAP and alphaCTD is small. These findings are consistent with the proposal that activation involves a simple "recruitment" mechanism.
- Publication
Science (New York, N.Y.), 2002, Vol 297, Issue 5586, p1562
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1076376