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- Title
Excess polymorphisms in genes for membrane proteins in Plasmodium falciparum.
- Authors
Volkman, Sarah K; Hartl, Daniel L; Wirth, Dyann F; Nielsen, Kaare M; Choi, Mehee; Batalov, Serge; Zhou, Yingyao; Plouffe, David; Le Roch, Karine G; Abagyan, Ruben; Winzeler, Elizabeth A
- Abstract
The detection of single-nucleotide polymorphisms in pathogenic microorganisms has normally been carried out by trial and error. Here we show that DNA hybridization with high-density oligonucleotide arrays provides rapid and convenient detection of single-nucleotide polymorphisms in Plasmodium falciparum, despite its exceptionally high adenine-thymine (AT) content (82%). A disproportionate number of polymorphisms are found in genes encoding proteins associated with the cell membrane. These genes are targets for only 22% of the oligonucleotide probes but account for 69% of the polymorphisms. Genetic variation is also enriched in subtelomeric regions, which account for 22% of the chromosome but 76% of the polymorphisms.
- Publication
Science (New York, N.Y.), 2002, Vol 298, Issue 5591, p216
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1075642