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- Title
Functional annotation of a full-length Arabidopsis cDNA collection.
- Authors
Seki, Motoaki; Narusaka, Mari; Kamiya, Asako; Ishida, Junko; Satou, Masakazu; Sakurai, Tetsuya; Nakajima, Maiko; Enju, Akiko; Akiyama, Kenji; Oono, Youko; Muramatsu, Masami; Hayashizaki, Yoshihide; Kawai, Jun; Carninci, Piero; Itoh, Masayoshi; Ishii, Yoshiyuki; Arakawa, Takahiro; Shibata, Kazuhiro; Shinagawa, Akira; Shinozaki, Kazuo
- Abstract
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.
- Publication
Science (New York, N.Y.), 2002, Vol 296, Issue 5565, p141
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1071006