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- Title
Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.
- Authors
Bousso, Philippe; Bhakta, Nirav R; Lewis, Richard S; Robey, Ellen
- Abstract
Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.
- Publication
Science (New York, N.Y.), 2002, Vol 296, Issue 5574, p1876
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1070945