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- Title
A new UAG-encoded residue in the structure of a methanogen methyltransferase.
- Authors
Hao, Bing; Gong, Weimin; Ferguson, Tsuneo K; James, Carey M; Krzycki, Joseph A; Chan, Michael K
- Abstract
Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.
- Publication
Science (New York, N.Y.), 2002, Vol 296, Issue 5572, p1462
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1069556